Do It Yourself Biology Research, Past and Present

Depends on what you're doing. If you're doing PCR to screen a large number of transformants, or if you're using the dNTPs to synthesize your own oligos, you'll go through a lot.

IMO, this is where the focus of DIY bio should be for a while. Try and find out how to get expensive reagents for reasonable prices. dNTPs are just one example. What are these people staining their gels with anyway? Ethidium bromide is somewhat toxic, and Sybr safe is fairly expensive.

Also, are people doing histology at home? You might be able to produce your own antibodies, if you keep rabbits. But can you conjugate fluorescent markers to them?

What about western blots? Is polyacrylamide available to the hobbyist?

I guess what I'm saying is that there's a lot that can be done, but also a lot that can't be done at home on the cheap. We're still in the phase where the most important research is on techniques, not biology.

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